ht 29 atcc cat Search Results


ht 1080  (ATCC)
98
ATCC ht 1080
Ht 1080, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htb63 human melanoma cell line  (ATCC)
95
ATCC htb63 human melanoma cell line
( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
Htb63 Human Melanoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dulbecco’s modified eagle’s medium (dmem)  (Thermo Fisher)
90
Thermo Fisher dulbecco’s modified eagle’s medium (dmem)
( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
Dulbecco’s Modified Eagle’s Medium (Dmem), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ht29  (ATCC)
99
ATCC ht29
( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
Ht29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ccl 247 ht 29 atcc cat n  (ATCC)
99
ATCC ccl 247 ht 29 atcc cat n
( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
Ccl 247 Ht 29 Atcc Cat N, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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catalog number 10536 cell reports 29  (ATCC)
99
ATCC catalog number 10536 cell reports 29
( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
Catalog Number 10536 Cell Reports 29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htc116  (DSMZ)
95
DSMZ htc116
( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, <t>HTB63,</t> and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.
Htc116, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p517 ht  (ATCC)
97
ATCC p517 ht
KEY RESOURCES TABLE
P517 Ht, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ht 29 cells  (ATCC)
96
ATCC ht 29 cells
KEY RESOURCES TABLE
Ht 29 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colorectal adenocarcinoma cell line ht 29  (ATCC)
99
ATCC colorectal adenocarcinoma cell line ht 29
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Colorectal Adenocarcinoma Cell Line Ht 29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human bladder cancer cell lines  (ATCC)
96
ATCC human bladder cancer cell lines
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Human Bladder Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human bladder cancer cell lines - by Bioz Stars, 2026-03
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ht supplement 100  (ATCC)
90
ATCC ht supplement 100
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Ht Supplement 100, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.

Journal: Oncotarget

Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion

doi: 10.18632/oncotarget.9332

Figure Lengend Snippet: ( A , B ) The panels show the gene expression of WNT5A and IL-6 in multiple microarray data sets of melanoma cell lines classified by the Heuristic Online Phenotype Prediction (HOPP) algorithm as proliferative versus invasive. ( C ) QPCR analyses of endogenous WNT5A mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression, and the results are presented relative to the WNT5A mRNA expression of WM852 cells ( n = 5). ( D ) Western blot analysis showing the levels of endogenous WNT5A protein expression in the human melanoma cell lines WM852, HTB63, and A375. α-tubulin was used as a loading control. A representative blot from four separate experiments is shown. The lower panel shows densitometric analyses of WNT5A protein expression normalised against α-tubulin; the results are presented relative to WNT5A protein expression of WM852 cells ( n = 4). ( E ) QPCR analysis of endogenous IL-6 mRNA expression levels in human WM852, HTB63, and A375 cells. Samples were normalised against TATA-binding protein (TBP) mRNA expression; the results are presented relative to the IL-6 mRNA expression of WM852 cells ( n = 5). ( F ) ELISA analysis showing the quantification of endogenous IL-6 protein secretion in human WM852, HTB63, and A375 cells ( n = 5). The results are presented as the means and S.E.Ms; *** p < 0.001.

Article Snippet: Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.

Techniques: Gene Expression, Microarray, Expressing, Binding Assay, Western Blot, Control, Enzyme-linked Immunosorbent Assay

Matrigel invasion of WM852 ( A and B ) and HTB63 ( C and D ) cells treated with either Box5 or neutralising anti-IL-6 antibody alone and their combination was evaluated as described under the Materials and Methods section. Proper controls (DMSO for Box5 treatment or IgG 1 isotype antibody for neutralising anti-IL-6-antibody treatment) were used to compare the treatment effect on the cell invasions. The invasion assays ( n = 5) were performed over 24 h and analysed as described in the Materials and Methods section. The results are presented as the means and S.E.Ms; * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Oncotarget

Article Title: Demonstration of a WNT5A-IL-6 positive feedback loop in melanoma cells: Dual interference of this loop more effectively impairs melanoma cell invasion

doi: 10.18632/oncotarget.9332

Figure Lengend Snippet: Matrigel invasion of WM852 ( A and B ) and HTB63 ( C and D ) cells treated with either Box5 or neutralising anti-IL-6 antibody alone and their combination was evaluated as described under the Materials and Methods section. Proper controls (DMSO for Box5 treatment or IgG 1 isotype antibody for neutralising anti-IL-6-antibody treatment) were used to compare the treatment effect on the cell invasions. The invasion assays ( n = 5) were performed over 24 h and analysed as described in the Materials and Methods section. The results are presented as the means and S.E.Ms; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Both the HTB63 human melanoma cell line (Cat#HT-144, LOT: 59550354) and the A375 human melanoma cell line (Cat#CRL-1619, LOT:61573377) were obtained from American Type Culture Collection (ATCC, VA, USA) in August 2014.

Techniques:

KEY RESOURCES TABLE

Journal: Developmental cell

Article Title: Cancer Cells Upregulate NRF2 Signaling to Adapt to Autophagy Inhibition

doi: 10.1016/j.devcel.2019.07.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies are listed in the . table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NRF2 Abcam Cat# ab62352; RRID: AB_944418 20-s Proteasome UBPBio Y2010 P62 Novus Cat# H00008878-M01; RRID: AB_548364 LC3 Novus Cat# NB100-2220; RRID: AB_10003146 ATG5 Cell Signaling Technology Cat# 9980S; RRID: AB_10829153 ATG7 Cell Signaling Technology Cat# 8558; RRID: AB_10831194 ATG12 Cell Signaling Technology Cat # 2010s; RRID: AB_2059086 STX17 Sigma-Aldrich Cat# HPA001204; RRID: AB_1080118 FIP200 Novus Cat# NBP1-31583; RRID: AB_2300812 PTEN Santa Cruz Biotechnology Cat# sc-7974; RRID: AB_628187 FOXO3a Cell Signaling Cat# 3938; RRID: AB_2106669 β-Actin Sigma-Aldrich Cat# A5441; RRID: AB_476744 β-Tubulin Sigma-Aldrich Cat# T5168; RRID: AB_477579 Mouse-IgG Cell Signaling Technology Cat# 7076; RRID: AB_330924 Rabbit-IgG Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Chemicals, Peptides, and Recombinant Proteins Bafilomycin A1 Sigma-Aldrich B1792; CAS RN: 88899-55-2 Chloroquine MP-Biomedicals 93919; CAS RN: 50-63-5 Protease Inhibitor Cocktail Roche 11836153001 Bortezomib Selleck Chemicals S1013 Critical Commercial Assays MEGAscript T7 Transcription Kit Thermo Fisher AM1354 MEGclear Transcription Clean Up Kit Thermo Fisher AM1908 Wizard SV Gel PCR clean up kit Promega A9282 Lipofectamine CRISPR Max Cas9 transfection reagent Thermo Fisher CMAX00003 RNeasy RNA isolation kit Qiagen 74104 QuantiTect Reverse Transcription Kit Qiagen 205311 SYBR Green CFX for QPCR Applied Biosystems Applied Biosystems 4472942 Seahorse XF Mitochondrial Stress Test Agilent 103015–100 Proteasome Activity Fluorometric Assay Kit II UBPBio J4120 CellROX green flow cytometry assay kit Thermo Fisher {"type":"entrez-nucleotide","attrs":{"text":"C10492","term_id":"1535563","term_text":"C10492"}} C10492 Experimental Models: Cell Lines HCT116 ATCC NCI-DTP Cat# HCT-116; RRID: CVCL_0291 MCF7 ATCC NCI-DTP Cat# MCF7; RRID: CVCL_0031 HT1080 ATCC CLS Cat# 300216/p517_HT-1080; RRID: CVCL_0317 NCIH292 Kindly provided from Dr. Joaquin Espinosa ECACC Cat# 91091815; RRID: CVCL_0455 MAF-794 Kindly provided from Dr. Jean Mulcahy-Levy N/A BT549 ATCC NCI-DTP Cat# BT-549; RRID: CVCL_1092 SJSA-1 Kindly provided from Dr. Joaquin Espinosa ATCC Cat# CRL-2098; RRID: CVCL_1697 NCIH-1650 ATCC ATCC Cat# CRL-5883; RRID: CVCL_1483 Experimental Models: Organisms/Strains Athymic Nude mice (nu/nu) Jackson Laboratory Stock number: 007850 Oligonucleotides Oligonucleotides for qRT-PCR primers: Table S1 this study; Fitzwalter et al., 2018 ; Oslowski and Urano, 2011 ; Radhakrishnan et al., 2010 ; Vangala et al., 2014 .

Techniques: Recombinant, Protease Inhibitor, CRISPR, Transfection, Isolation, SYBR Green Assay, Activity Assay, Flow Cytometry, shRNA, Plasmid Preparation